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In vitro and in vivo two-photon luminescence imaging of single gold nanorods.

ABSTRACT: Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as TPL imaging agents is demonstrated by in vivo imaging of single nanorods flowing in mouse ear blood vessels.

SUBMITTER: Wang H 

PROVIDER: S-EPMC1276057 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature

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In vitro and in vivo two-photon luminescence imaging of single gold nanorods.

Wang Haifeng H   Huff Terry B TB   Zweifel Daniel A DA   He Wei W   Low Philip S PS   Wei Alexander A   Cheng Ji-Xin JX  

Proceedings of the National Academy of Sciences of the United States of America 20051020 44

Gold nanorods excited at 830 nm on a far-field laser-scanning microscope produced strong two-photon luminescence (TPL) intensities, with a cos(4) dependence on the incident polarization. The TPL excitation spectrum can be superimposed onto the longitudinal plasmon band, indicating a plasmon-enhanced two-photon absorption cross section. The TPL signal from a single nanorod is 58 times that of the two-photon fluorescence signal from a single rhodamine molecule. The application of gold nanorods as  ...[more]

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