Ontology highlight
ABSTRACT:
SUBMITTER: Ueki H
PROVIDER: S-EPMC7086515 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Ueki Hiroshi H Wang I-Hsuan IH Zhao Dongming D Gunzer Matthias M Kawaoka Yoshihiro Y
Nature protocols 20200129 3
In vivo two-photon imaging is a valuable technique for studies of viral pathogenesis and host responses to infection in vivo. In this protocol, we describe a methodology for analyzing influenza virus-infected lung in vivo by two-photon imaging microscopy. We describe the surgical procedure, how to stabilize the lung, and an approach to analyzing the data. Further, we provide a database of fluorescent dyes, antibodies, and reporter mouse lines that can be used in combination with a reporter influ ...[more]