Ontology highlight
ABSTRACT:
SUBMITTER: Ignatov A
PROVIDER: S-EPMC127865 | biostudies-literature | 2002 Apr
REPOSITORIES: biostudies-literature
Ignatov Atanas A Keath Elizabeth J EJ
Infection and immunity 20020401 4
A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens. ...[more]