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Novel 33-kilodalton lipoprotein from Mycobacterium leprae.

ABSTRACT: A novel Mycobacterium leprae lipoprotein LpK (accession no. ML0603) was identified from the genomic database. The 1,116-bp open reading frame encodes a 371-amino-acid precursor protein with an N-terminal signal sequence and a consensus motif for lipid conjugation. Expression of the protein, LpK, in Escherichia coli revealed a 33-kDa protein, and metabolic labeling experiments and globomycin treatment proved that the protein was lipidated. Fractionation of M. leprae demonstrated that this lipoprotein was a membrane protein of M. leprae. The purified lipoprotein was found to induce production of interleukin-12 in human peripheral blood monocytes. The studies imply that M. leprae LpK is involved in protective immunity against leprosy and may be a candidate for vaccine design.

SUBMITTER: Maeda Y

PROVIDER: S-EPMC128180 | biostudies-literature | 2002 Aug

REPOSITORIES: biostudies-literature

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Novel 33-kilodalton lipoprotein from Mycobacterium leprae.

Maeda Yumi Y Makino Masahiko M Crick Dean C DC Mahapatra Sebabrata S Srisungnam Sopa S Takii Takemasa T Kashiwabara Yoshiko Y Brennan Patrick J PJ

Infection and immunity 20020801 8

A novel Mycobacterium leprae lipoprotein LpK (accession no. ML0603) was identified from the genomic database. The 1,116-bp open reading frame encodes a 371-amino-acid precursor protein with an N-terminal signal sequence and a consensus motif for lipid conjugation. Expression of the protein, LpK, in Escherichia coli revealed a 33-kDa protein, and metabolic labeling experiments and globomycin treatment proved that the protein was lipidated. Fractionation of M. leprae demonstrated that this lipopro ...[more]

PMID: 12117918

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