Ontology highlight
ABSTRACT:
SUBMITTER: Zhang MX
PROVIDER: S-EPMC1287968 | biostudies-literature | 2005 Nov
REPOSITORIES: biostudies-literature
Zhang Ming-Xiang MX Ou Hesheng H Shen Ying H YH Wang Jing J Wang Jian J Coselli Joseph J Wang Xing Li XL
Proceedings of the National Academy of Sciences of the United States of America 20051111 47
Repeats (27-nt) in intron 4 have been shown to play a cis-acting role in endothelial nitric oxide synthase (eNOS) promoter activity. We hypothesize that the 27-nt repeats could be the source of small nuclear RNA specifically regulating eNOS expression. In this study, we used synthesized 27-nt RNA duplex and found that the eNOS gene transcriptional efficiency was reduced 63% (0.047 +/- 0.009 vs. 0.126 +/- 0.015, P < 0.01) by nuclear run-on assay. In endothelial cells transfected with the 27-nt sm ...[more]