Unknown

Dataset Information

0

The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants.


ABSTRACT: Replication forks arrested by inactivation of the main Escherichia coli DNA polymerase (polymerase III) are reversed by the annealing of newly synthesized leading- and lagging-strand ends. Reversed forks are reset by the action of RecBC on the DNA double-strand end, and in the absence of RecBC chromosomes are linearized by the Holliday junction resolvase RuvABC. We report here that the UvrD helicase is essential for RuvABC-dependent chromosome linearization in E. coli polymerase III mutants, whereas its partners in DNA repair (UvrA/B and MutL/S) are not. We conclude that UvrD participates in replication fork reversal in E. coli.

SUBMITTER: Flores MJ 

PROVIDER: S-EPMC1299159 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants.

Flores Maria Jose MJ   Bidnenko Vladimir V   Michel Bénédicte B  

EMBO reports 20040917 10


Replication forks arrested by inactivation of the main Escherichia coli DNA polymerase (polymerase III) are reversed by the annealing of newly synthesized leading- and lagging-strand ends. Reversed forks are reset by the action of RecBC on the DNA double-strand end, and in the absence of RecBC chromosomes are linearized by the Holliday junction resolvase RuvABC. We report here that the UvrD helicase is essential for RuvABC-dependent chromosome linearization in E. coli polymerase III mutants, whe  ...[more]

Similar Datasets

| S-EPMC6086610 | biostudies-literature
| S-EPMC3320595 | biostudies-literature
| S-EPMC6166810 | biostudies-literature
| S-EPMC2034406 | biostudies-literature
| S-EPMC1383526 | biostudies-other
| S-EPMC4260453 | biostudies-literature
| S-EPMC4362724 | biostudies-literature
| S-EPMC8452682 | biostudies-literature
| S-EPMC5643541 | biostudies-literature
| S-EPMC4351167 | biostudies-literature