Ontology highlight
ABSTRACT:
SUBMITTER: Kato M
PROVIDER: S-EPMC1303095 | biostudies-literature | 2003 Jul
REPOSITORIES: biostudies-literature
Kato Mikio M Hokabe Shingo S Itakura Shuji S Minoshima Shinsei S Lyubchenko Yuri L YL Gurkov Theodor D TD Okawara Hiroshi H Nagayama Kuniaki K Shimizu Nobuyoshi N
Biophysical journal 20030701 1
A novel interarm interaction of DNA cruciform forming at inverted repeat sequence was characterized using an S1 nuclease digestion, permanganate oxidation, and microscopic imaging. An inverted repeat consisting of 17 bp complementary sequences was isolated from the bluegill sunfish Lepomis macrochirus (Perciformes) and subcloned into the pUC19 plasmid, after which the supercoiled recombinant plasmid was subjected to enzymatic and chemical modification. In high salt conditions (200 mM NaCl, or 10 ...[more]