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Characterization of the DNA gyrase from the thermoacidophilic archaeon Thermoplasma acidophilum.

ABSTRACT: Thermoplasma acidophilum is sensitive to the antibiotic drug novobiocin, which inhibits DNA gyrase. We characterized DNA gyrases from T. acidophilum strains in vitro. The DNA gyrase from a novobiocin-resistant strain and an engineered mutant were less sensitive to novobiocin. The novobiocin-resistant gyrase genes might serve as T. acidophilum genetic markers.

SUBMITTER: Yamashiro K

PROVIDER: S-EPMC1316996 | biostudies-literature | 2005 Dec

REPOSITORIES: biostudies-literature

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Characterization of the DNA gyrase from the thermoacidophilic archaeon Thermoplasma acidophilum.

Yamashiro Kan K Yamagishi Akihiko A

Journal of bacteriology 20051201 24

Thermoplasma acidophilum is sensitive to the antibiotic drug novobiocin, which inhibits DNA gyrase. We characterized DNA gyrases from T. acidophilum strains in vitro. The DNA gyrase from a novobiocin-resistant strain and an engineered mutant were less sensitive to novobiocin. The novobiocin-resistant gyrase genes might serve as T. acidophilum genetic markers. ...[more]

PMID: 16321962

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