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Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum.

ABSTRACT: Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM.

SUBMITTER: Haugland GT

PROVIDER: S-EPMC2627522 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature

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Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum.

Haugland Gyri Teien GT Innselset Marte M Madern Dominique D Birkeland Nils-Kåre NK

The open biochemistry journal 20080927

Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity ...[more]

PMID: 19238189

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