Unknown

Dataset Information

0

Functional analyses of RNA structures shared between the internal ribosome entry sites of hepatitis C virus and the picornavirus porcine teschovirus 1 Talfan.


ABSTRACT: The internal ribosome entry site (IRES) of porcine teschovirus 1 (PTV-1), a member of the Picornaviridae family, is quite distinct from other well-characterized picornavirus IRES elements, but it displays functional similarities to the IRES from hepatitis C virus (HCV), a member of the Flaviviridae family. In particular, a dominant negative mutant form of eIF4A does not inhibit the activity of the PTV-1 IRES. Furthermore, there is a high level (ca. 50%) of identity between the PTV-1 and HCV IRES sequences. A secondary-structure model of the whole PTV-1 IRES has been derived which includes a pseudoknot. Validation of specific features within the model has been achieved by mutagenesis and functional assays. The differences and similarities between the PTV-1 and HCV IRES elements should assist in defining the critical features of this type of IRES.

SUBMITTER: Chard LS 

PROVIDER: S-EPMC1346926 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Functional analyses of RNA structures shared between the internal ribosome entry sites of hepatitis C virus and the picornavirus porcine teschovirus 1 Talfan.

Chard Louisa S LS   Kaku Yoshihiro Y   Jones Barbara B   Nayak Arabinda A   Belsham Graham J GJ  

Journal of virology 20060201 3


The internal ribosome entry site (IRES) of porcine teschovirus 1 (PTV-1), a member of the Picornaviridae family, is quite distinct from other well-characterized picornavirus IRES elements, but it displays functional similarities to the IRES from hepatitis C virus (HCV), a member of the Flaviviridae family. In particular, a dominant negative mutant form of eIF4A does not inhibit the activity of the PTV-1 IRES. Furthermore, there is a high level (ca. 50%) of identity between the PTV-1 and HCV IRES  ...[more]

Similar Datasets

| S-EPMC136790 | biostudies-literature
| S-EPMC5727462 | biostudies-literature
| S-EPMC3255860 | biostudies-literature
| S-EPMC2258716 | biostudies-literature
| S-EPMC1950536 | biostudies-literature
| S-EPMC4106463 | biostudies-literature
| S-EPMC3592162 | biostudies-literature
| S-EPMC1084086 | biostudies-literature
| S-EPMC1456917 | biostudies-literature
| S-EPMC6664791 | biostudies-literature