Unknown

Dataset Information

0

Proteome and transcriptome analysis of the virulence genes regulated by the VirR/VirS system in Clostridium perfringens.


ABSTRACT: The proteins under the control of the two-component system VirR/VirS in Clostridium perfringens were analyzed by using two-dimensional gel electrophoresis of the culture supernatant from the wild type and the virR mutant. Based on matrix-assisted laser desorption ionization-time of flight/mass spectrometry, seven positively regulated proteins and eight negatively regulated proteins were identified. Transcriptome analysis confirmed that 7 of the 15 proteins were regulated by the VirR/VirS system at the transcriptional level, but the remaining proteins were modified with a VirR/VirS-directed protease at the posttranslation and secretion levels. We purified and characterized the VirR/VirS-directed protease from the culture supernatant and identified it as a kind of clostripain. Because this proteolytic activity was strongly inhibited by leupeptin and antipain, it was concluded that this protease was a member of the family of cysteine proteases of C. perfringens.

SUBMITTER: Shimizu T 

PROVIDER: S-EPMC135029 | biostudies-literature | 2002 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Proteome and transcriptome analysis of the virulence genes regulated by the VirR/VirS system in Clostridium perfringens.

Shimizu Takeshi T   Shima Kensuke K   Yoshino Ken-ichi K   Yonezawa Kazuyoshi K   Shimizu Tohru T   Hayashi Hideo H  

Journal of bacteriology 20020501 10


The proteins under the control of the two-component system VirR/VirS in Clostridium perfringens were analyzed by using two-dimensional gel electrophoresis of the culture supernatant from the wild type and the virR mutant. Based on matrix-assisted laser desorption ionization-time of flight/mass spectrometry, seven positively regulated proteins and eight negatively regulated proteins were identified. Transcriptome analysis confirmed that 7 of the 15 proteins were regulated by the VirR/VirS system  ...[more]

Similar Datasets

| S-EPMC4877802 | biostudies-literature
2016-08-03 | GSE72137 | GEO
| S-EPMC2838878 | biostudies-other
2016-08-03 | E-GEOD-72137 | biostudies-arrayexpress
2009-09-17 | GSE12833 | GEO
| S-EPMC3236063 | biostudies-literature
| S-EPMC1865775 | biostudies-literature
2010-05-05 | E-GEOD-12833 | biostudies-arrayexpress
| S-EPMC4886753 | biostudies-literature
| S-EPMC1456398 | biostudies-literature