VirR and RevR regulate many Clostridium perfringens genes in a reciprocal manner

ABSTRACT: We further characterize the VirSR and RevR regulatory networks by profiling the C. perfringens strain JIR325 and its isogenic virR and revR regulatory mutants using strand-specific RNA-seq. Two independent biological replicates were sequenced for each strain, generating more than 90 million sequence reads for each RNA-seq library (wild type, 97,289,148 reads; virR mutant, 116,505,992 reads and revR mutant, 131,811,486 reads). Using the edgeR analysis package, 223 genes in the virR mutant and 88 genes in the revR mutant were found to be differentially expressed. Comparative transcriptomic analysis revealed that VirR acts as a global negative regulator, whilst RevR acts as a global positive regulator. Therefore, about 88% of the differentially expressed genes were up-regulated in the virR mu

ORGANISM(S): Clostridium perfringens

SUBMITTER: Julian Rood 

PROVIDER: E-GEOD-72137 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress