The Rhizobium etli cyaC product: characterization of a novel adenylate cyclase class.
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ABSTRACT:
SUBMITTER: Tellez-Sosa J
PROVIDER: S-EPMC135151 | biostudies-literature | 2002 Jul
REPOSITORIES: biostudies-literature
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The Rhizobium etli cyaC product: characterization of a novel adenylate cyclase class.
Téllez-Sosa Juan J Soberón Nora N Vega-Segura Alicia A Torres-Márquez María E ME Cevallos Miguel A MA
Journal of bacteriology 20020701 13
Adenylate cyclases (ACs) catalyze the formation of 3',5'-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC, was isolated from Rhizobium etli by phenotypic complementation of an Escherichia coli cya mutant. The functionality of the cyaC gene was corroborated by its ability to restore cAMP accumulation in an E. coli cya mutant. Further, overexpression of a malE::cyaC fusion protein allowed the detection of significant AC activity levels in cell extracts of an E. coli cya mutant. CyaC is u ...[more]
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