Ontology highlight
ABSTRACT:
SUBMITTER: Raddatz S
PROVIDER: S-EPMC135808 | biostudies-literature | 2002 Nov
REPOSITORIES: biostudies-literature
Raddatz Stefan S Mueller-Ibeler Jochen J Kluge Joachim J Wäss Ludger L Burdinski Gerhard G Havens John R JR Onofrey Tom J TJ Wang Daguang D Schweitzer Markus M
Nucleic acids research 20021101 21
We report the synthesis of new phosphoramidite building blocks and their use for the modification of oligonucleotides with hydrazides. The reaction of these hydrazide oligonucleotides with active esters and aldehydes is demonstrated for solution conjugation and immobilization. Compared with the established amino modified oligonucleotides, hydrazides show enhanced reactivity at neutral and acidic buffer conditions. One method to introduce hydrazides is using amidites with preformed, protected hyd ...[more]