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Generation of a chemical gradient across an array of 256 cell cultures in a single chip.

ABSTRACT: A microfluidic diffusion diluter to create stable chemical gradients across an array of cell cultures was demonstrated. The device enabled concentration based studies to be conducted at 256 different concentrations across individual, low shear cell cultures. A gradient of staurosporine on cells stained with Mitotracker Deep Red (MTDR) showed a concentration-based effect on cell apoptosis across the cell culture array.

SUBMITTER: Somaweera H

PROVIDER: S-EPMC3857027 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

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Generation of a chemical gradient across an array of 256 cell cultures in a single chip.

Somaweera Himali H Ibragimov Akif A Pappas Dimitri D

The Analyst 20131001 19

A microfluidic diffusion diluter to create stable chemical gradients across an array of cell cultures was demonstrated. The device enabled concentration based studies to be conducted at 256 different concentrations across individual, low shear cell cultures. A gradient of staurosporine on cells stained with Mitotracker Deep Red (MTDR) showed a concentration-based effect on cell apoptosis across the cell culture array. ...[more]

PMID: 23939026

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