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Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae.


ABSTRACT: Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p.

SUBMITTER: Van Dellen KL 

PROVIDER: S-EPMC1360259 | biostudies-literature | 2006 Jan

REPOSITORIES: biostudies-literature

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Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae.

Van Dellen Katrina L KL   Bulik Dorota A DA   Specht Charles A CA   Robbins Phillips W PW   Samuelson John C JC  

Eukaryotic cell 20060101 1


Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p. ...[more]

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