Ontology highlight
ABSTRACT:
SUBMITTER: Thumfart JO
PROVIDER: S-EPMC136211 | biostudies-literature | 2002 Jun
REPOSITORIES: biostudies-literature
Thumfart Jörg Oliver JO Meyers Gregor G
Journal of virology 20020601 12
The RNA genome of the vaccine strain 2024 of feline calicivirus was cloned as cDNA and analyzed by nucleotide sequencing. A full-length DNA copy of the viral genome was established and proved to be a source of infectious cRNA after in vitro transcription and RNA transfection. Virus could also be recovered when the DNA construct was introduced into cells containing phage T7 RNA polymerase that was provided by vaccinia virus MVA-T7. After insertion of the sequence encoding the green fluorescent pr ...[more]