Aspergillus fumigatus C-5 sterol desaturases Erg3A and Erg3B: role in sterol biosynthesis and antifungal drug susceptibility.
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ABSTRACT:
SUBMITTER: Alcazar-Fuoli L
PROVIDER: S-EPMC1366924 | biostudies-literature | 2006 Feb
REPOSITORIES: biostudies-literature
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Aspergillus fumigatus C-5 sterol desaturases Erg3A and Erg3B: role in sterol biosynthesis and antifungal drug susceptibility.
Alcazar-Fuoli Laura L Mellado Emilia E Garcia-Effron Guillermo G Buitrago Maria J MJ Lopez Jordi F JF Grimalt Joan O JO Cuenca-Estrella J Manuel JM Rodriguez-Tudela Juan L JL
Antimicrobial agents and chemotherapy 20060201 2
Two erg3 genes encoding C-5 sterol desaturase enzymes (Erg3A and Erg3B) in Aspergillus fumigatus were characterized with respect to their nucleotide sequences and null mutant phenotypes. Targeted disruption of the erg3A and erg3B genes and a double gene knockout, erg3A- erg3B-, showed that they are not essential for A. fumigatus viability. Mutant phenotypes clearly showed that Erg3B is a C-5 sterol desaturase, but no apparent role for Erg3A in A. fumigatus ergosterol biosynthesis was found. Susc ...[more]
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