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Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd.


ABSTRACT: We used cryo-electron microscopy and image reconstruction to investigate the structure and microtubule-binding configurations of dimeric non-claret disjunctional (ncd) motor domains under various nucleotide conditions, and applied molecular docking using ncd's dimeric X-ray structure to generate a mechanistic model for force transduction. To visualize the alpha-helical coiled-coil neck better, we engineered an SH3 domain to the N-terminal end of our ncd construct (296-700). Ncd exhibits strikingly different nucleotide-dependent three-dimensional conformations and microtubule-binding patterns from those of conventional kinesin. In the absence of nucleotide, the neck adapts a configuration close to that found in the X-ray structure with stable interactions between the neck and motor core domain. Minus-end-directed movement is based mainly on two key events: (i) the stable neck-core interactions in ncd generate a binding geometry between motor and microtubule which places the motor ahead of its cargo in the minus-end direction; and (ii) after the uptake of ATP, the two heads rearrange their position relative to each other in a way that promotes a swing of the neck in the minus-end direction.

SUBMITTER: Wendt TG 

PROVIDER: S-EPMC137211 | biostudies-literature | 2002 Nov

REPOSITORIES: biostudies-literature

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Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd.

Wendt Thomas G TG   Volkmann Niels N   Skiniotis Georgios G   Goldie Kenneth N KN   Müller Jens J   Mandelkow Eckhard E   Hoenger Andreas A  

The EMBO journal 20021101 22


We used cryo-electron microscopy and image reconstruction to investigate the structure and microtubule-binding configurations of dimeric non-claret disjunctional (ncd) motor domains under various nucleotide conditions, and applied molecular docking using ncd's dimeric X-ray structure to generate a mechanistic model for force transduction. To visualize the alpha-helical coiled-coil neck better, we engineered an SH3 domain to the N-terminal end of our ncd construct (296-700). Ncd exhibits striking  ...[more]

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