Ontology highlight
ABSTRACT:
SUBMITTER: Wendt TG
PROVIDER: S-EPMC137211 | biostudies-literature | 2002 Nov
REPOSITORIES: biostudies-literature
Wendt Thomas G TG Volkmann Niels N Skiniotis Georgios G Goldie Kenneth N KN Müller Jens J Mandelkow Eckhard E Hoenger Andreas A
The EMBO journal 20021101 22
We used cryo-electron microscopy and image reconstruction to investigate the structure and microtubule-binding configurations of dimeric non-claret disjunctional (ncd) motor domains under various nucleotide conditions, and applied molecular docking using ncd's dimeric X-ray structure to generate a mechanistic model for force transduction. To visualize the alpha-helical coiled-coil neck better, we engineered an SH3 domain to the N-terminal end of our ncd construct (296-700). Ncd exhibits striking ...[more]