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Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.

ABSTRACT: The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample.

SUBMITTER: Hunt DE

PROVIDER: S-EPMC1393206 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature

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Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.

Hunt Dana E DE Klepac-Ceraj Vanja V Acinas Silvia G SG Gautier Clement C Bertilsson Stefan S Polz Martin F MF Polz Martin F MF

Applied and environmental microbiology 20060301 3

The availability of a diverse set of 23S rRNA gene sequences enabled evaluation of the specificity of 39 previously published and 4 newly designed primers specific for bacteria. An extensive clone library constructed using an optimized primer pair resulted in similar gene richness but slightly differing coverage of some phylogenetic groups, compared to a 16S rRNA gene library from the same environmental sample. ...[more]

PMID: 16517676

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