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Single-feature inking and stamping: a versatile approach to molecular patterning.

ABSTRACT: A technique for micrometer-scale patterning of multiple functional biological molecules on surfaces is demonstrated. The technique is referred to as single-feature inking and stamping (SFINKS). It combines elements of dip-pen nanolithography and microcontact printing. "Inked" atomic force microscopy probes are used to ink individual features of an elastomer stamp. From a single stamp, we printed three different probe ssDNA with <10 mum resolution and showed that they specifically hybridize the complementary DNA labeled with different fluorophores. As a further demonstration of SFINKS' versatility, we patterned a silane onto a silicon wafer consisting of four subpatterns separated by >100 mum and composed of 2 mum lines. We discuss why patterns such as these are impractical with available techniques. Furthermore, we comment on the prospects for multiple stamping after a single inking.

SUBMITTER: Gerding JD

PROVIDER: S-EPMC1395502 | biostudies-literature | 2005 Feb

REPOSITORIES: biostudies-literature

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Single-feature inking and stamping: a versatile approach to molecular patterning.

Gerding Jonathon D JD Willard Dale M DM Van Orden Alan A

Journal of the American Chemical Society 20050201 4

A technique for micrometer-scale patterning of multiple functional biological molecules on surfaces is demonstrated. The technique is referred to as single-feature inking and stamping (SFINKS). It combines elements of dip-pen nanolithography and microcontact printing. "Inked" atomic force microscopy probes are used to ink individual features of an elastomer stamp. From a single stamp, we printed three different probe ssDNA with <10 mum resolution and showed that they specifically hybridize the c ...[more]

PMID: 15669842

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Dataset Information

Single-feature inking and stamping: a versatile approach to molecular patterning.

Publications

Single-feature inking and stamping: a versatile approach to molecular patterning.

Similar Datasets

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