Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis.
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ABSTRACT:
SUBMITTER: Woodford N
PROVIDER: S-EPMC139696 | biostudies-literature | 2002 Nov
REPOSITORIES: biostudies-literature
Publications
Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis.
Woodford Neil N Tysall Luke L Auckland Cressida C Stockdale Mark W MW Lawson Andrew J AJ Walker Rachel A RA Livermore David M DM
Journal of clinical microbiology 20021101 11
A real-time PCR assay identified linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolates with a G2576U rRNA mutation. PCR-restriction fragment length polymorphism analysis of ribosomal DNA amplicons with NheI also detected this mutation. Both assays detected isolates heterozygous at this position. Recognition of isolates with what is presently the most frequent oxazolidinone resistance mutation may aid surveillance and individual case management. ...[more]
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