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Atypical URA5 gene restriction fragment length polymorphism banding profile in Cryptococcus neoformans strains.


ABSTRACT: URA5-RFLP is one of the most widely used genotyping methods relating to Cryptococcus neoformans and C. gattii consensus genotype nomenclature. In order to identify a molecular type, this method uses a visual comparison of digested PCR products of tested and reference strains, therefore any anomaly in RFLP patterns of studied isolates makes recognition difficult or impossible. This report describes a strain of VNIV type showing an atypical URA5-RFLP pattern as well as a group of AD hybrids displaying the same anomaly. The atypical RFLP pattern is the result of a point mutation and emergence of a new restriction site. Emergence of the allele presenting a new banding pattern may lead to misidentification using the URA5-RFLP technique; the results of this study as well as the literature data may suggest the spread of the allele in the environment.

SUBMITTER: Florek M 

PROVIDER: S-EPMC6861435 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature

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Atypical URA5 gene restriction fragment length polymorphism banding profile in Cryptococcus neoformans strains.

Florek Magdalena M   Król Jarosław J   Woźniak-Biel Anna A  

Folia microbiologica 20190408 6


URA5-RFLP is one of the most widely used genotyping methods relating to Cryptococcus neoformans and C. gattii consensus genotype nomenclature. In order to identify a molecular type, this method uses a visual comparison of digested PCR products of tested and reference strains, therefore any anomaly in RFLP patterns of studied isolates makes recognition difficult or impossible. This report describes a strain of VNIV type showing an atypical URA5-RFLP pattern as well as a group of AD hybrids displa  ...[more]

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