Unknown

Dataset Information

0

Solution structure of the PHD domain from the KAP-1 corepressor: structural determinants for PHD, RING and LIM zinc-binding domains.


ABSTRACT: Plant homeodomain (PHD) domains are found in >400 eukaryotic proteins, many of which are transcriptional regulators. Naturally occurring point mutations or deletions of this domain contribute to a variety of human diseases, including ATRX syndrome, myeloid leukemias and autoimmune dysfunction. Here we report the first structural characterization of a PHD domain. Our studies reveal that the PHD domain from KAP-1 corepressor binds zinc in a cross-brace topology between anti-parallel ss-strands reminiscent of RING (really interesting new gene) domains. Using a mutational analysis, we define the structural features required for transcriptional repression by KAP-1 and explain naturally occurring, disease-causing mutations in PHD domains of other proteins. From a comparison of this PHD structure with previously reported RING and LIM (Lin11/Isl-1/Mec-3) structures, we infer sequence determinants that allow discrimination among PHD, RING and LIM motifs.

SUBMITTER: Capili AD 

PROVIDER: S-EPMC140198 | biostudies-literature | 2001 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Solution structure of the PHD domain from the KAP-1 corepressor: structural determinants for PHD, RING and LIM zinc-binding domains.

Capili A D AD   Schultz D C DC   RauscherIII F J FJ   Borden K L KL  

The EMBO journal 20010101 1-2


Plant homeodomain (PHD) domains are found in >400 eukaryotic proteins, many of which are transcriptional regulators. Naturally occurring point mutations or deletions of this domain contribute to a variety of human diseases, including ATRX syndrome, myeloid leukemias and autoimmune dysfunction. Here we report the first structural characterization of a PHD domain. Our studies reveal that the PHD domain from KAP-1 corepressor binds zinc in a cross-brace topology between anti-parallel ss-strands rem  ...[more]

Similar Datasets

| S-EPMC8394524 | biostudies-literature
| S-EPMC87072 | biostudies-literature
| S-EPMC4919451 | biostudies-literature
| S-EPMC3793974 | biostudies-literature
| S-EPMC5913730 | biostudies-literature
| S-EPMC3543016 | biostudies-literature
| S-EPMC133676 | biostudies-literature
| S-EPMC5521587 | biostudies-literature
| S-EPMC8357790 | biostudies-literature
| S-EPMC6487577 | biostudies-literature