Improving baculovirus recombination.
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ABSTRACT:
SUBMITTER: Zhao Y
PROVIDER: S-EPMC140531 | biostudies-literature | 2003 Jan
REPOSITORIES: biostudies-literature
Publications
Improving baculovirus recombination.
Zhao Yuguang Y Chapman David A G DA Jones Ian M IM
Nucleic acids research 20030101 2
Recombinant baculoviruses have established themselves as a favoured technology for the high-level expression of recombinant proteins. The construction of recombinant viruses, however, is a time consuming step that restricts consideration of the technology for high throughput developments. Here we use a targeted gene knockout technology to inactivate an essential viral gene that lies adjacent to the locus used for recombination. Viral DNA prepared from the knockout fails to initiate an infection ...[more]
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