Ontology highlight
ABSTRACT:
SUBMITTER: Feng W
PROVIDER: S-EPMC1414058 | biostudies-literature | 2006 Feb
REPOSITORIES: biostudies-literature
Feng Wenyi W Collingwood David D Boeck Max E ME Fox Lindsay A LA Alvino Gina M GM Fangman Walton L WL Raghuraman Mosur K MK Brewer Bonita J BJ
Nature cell biology 20060122 2
During DNA replication one or both strands transiently become single stranded: first at the sites where initiation of DNA synthesis occurs (known as origins of replication) and subsequently on the lagging strands of replication forks as discontinuous Okazaki fragments are generated. We report a genome-wide analysis of single-stranded DNA (ssDNA) formation in the presence of hydroxyurea during DNA replication in wild-type and checkpoint-deficient rad53 Saccharomyces cerevisiae cells. In wild-type ...[more]