Ontology highlight
ABSTRACT:
SUBMITTER: St-Jean JR
PROVIDER: S-EPMC1440365 | biostudies-literature | 2006 Apr
REPOSITORIES: biostudies-literature
St-Jean Julien R JR Desforges Marc M Almazán Fernando F Jacomy Hélène H Enjuanes Luis L Talbot Pierre J PJ
Journal of virology 20060401 7
This study describes the assembly of a full-length cDNA clone of human coronavirus (HCoV)-OC43 in a bacterial artificial chromosome (BAC). The BAC containing the full-length infectious cDNA (pBAC-OC43(FL)) was assembled using a two-part strategy. The first step consisted in the introduction of each end of the viral genome into the BAC with accessory sequences allowing proper transcription. The second step consisted in the insertion of the whole HCoV-OC43 cDNA genome into the BAC. To produce reco ...[more]