Ontology highlight
ABSTRACT:
SUBMITTER: Gonzalez JM
PROVIDER: S-EPMC155106 | biostudies-literature | 2002 May
REPOSITORIES: biostudies-literature
Journal of virology 20020501 9
The stable propagation of a full-length transmissible gastroenteritis coronavirus (TGEV) cDNA in Escherichia coli cells as a bacterial artificial chromosome has been considerably improved by the insertion of an intron to disrupt a toxic region identified in the viral genome. The viral RNA was expressed in the cell nucleus under the control of the cytomegalovirus promoter and the intron was efficiently removed during translocation of this RNA to the cytoplasm. The insertion in two different posit ...[more]