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Use of semiconductor-based oligonucleotide microarrays for influenza a virus subtype identification and sequencing.

ABSTRACT: In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here we describe a genome assay and microarray design that can be used to rapidly identify influenza A virus hemagglutinin subtypes 1 through 15 and neuraminidase subtypes 1 through 9. Also described is an array-based enzymatic assay that can be used to sequence portions of both genes or any other sequence of interest.

SUBMITTER: Lodes MJ

PROVIDER: S-EPMC1448669 | biostudies-literature | 2006 Apr

REPOSITORIES: biostudies-literature

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Use of semiconductor-based oligonucleotide microarrays for influenza a virus subtype identification and sequencing.

Lodes Michael J MJ Suciu Dominic D Elliott Mark M Stover Axel G AG Ross Marty M Caraballo Marcelo M Dix Kim K Crye James J Webby Richard J RJ Lyon Wanda J WJ Danley David L DL McShea Andrew A

Journal of clinical microbiology 20060401 4

In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here we describe a genome assay and microarray design that can be used to rapidly identify influenza A virus hemagglutinin subtypes 1 through 15 and neuraminidase subtypes 1 through 9. ...[more]

PMID: 16597840

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