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Semiconductor based DNA sequencing of histone modification states

ABSTRACT: The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here, we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumor tissues.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE49477 | GEO | 2013/10/25

SECONDARY ACCESSION(S): PRJNA213998

REPOSITORIES: GEO

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Semiconductor based DNA sequencing of histone modification states

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