Ontology highlight
ABSTRACT:
SUBMITTER: Guarise C
PROVIDER: S-EPMC1449631 | biostudies-literature | 2006 Mar
REPOSITORIES: biostudies-literature
Guarise Cristian C Pasquato Lucia L De Filippis Vincenzo V Scrimin Paolo P
Proceedings of the National Academy of Sciences of the United States of America 20060301 11
We describe here a simple assay that allows the visual detection of a protease. The method takes advantage of the high molar absorptivity of the plasmon band of gold colloids and is based on the color change of their solution when treated with dithiols. We used C- and N-terminal cysteinyl derivatives of a peptide substrate exploiting its selective recognition and cleavage by a specific protease. Contrary to the native ones, cleaved peptides are unable to induce nanoparticles aggregation; hence, ...[more]