Dataset Information

Highly Sensitive Colorimetric Assay for Determining Fe³⁺ Based on Gold Nanoparticles Conjugated with Glycol Chitosan.

ABSTRACT: A highly sensitive and simple colorimetric assay for the detection of Fe³⁺ ions was developed using gold nanoparticles (AuNPs) conjugated with glycol chitosan (GC). The Fe³⁺ ion coordinates with the oxygen atoms of GC in a hexadentate manner (O-Fe³⁺-O), decreasing the interparticle distance and inducing aggregation. Time-of-flight secondary ion mass spectrometry showed that the bound Fe³⁺ was coordinated to the oxygen atoms of the ethylene glycol in GC, which resulted in a significant color change from light red to dark midnight blue due to aggregation. Using this GC-AuNP probe, the quantitative determination of Fe³⁺ in biological, environmental, and pharmaceutical samples could be achieved by the naked eye and spectrophotometric methods. Sensitive response and pronounced color change of the GC-AuNPs in the presence of Fe³⁺ were optimized at pH 6, 70°C, and 300?mM NaCl concentration. The absorption intensity ratio (A₇₀₀/A₅₁₀) linearly correlated to the Fe³⁺ concentration in the linear range of 0-180??M. The limits of detection were 11.3, 29.2, and 46.0?nM for tap water, pond water, and iron supplement tablets, respectively. Owing to its facile and sensitive nature, this assay method for Fe³⁺ ions can be applied to the analysis of drinking water and pharmaceutical samples.

SUBMITTER: Kim K

PROVIDER: S-EPMC5463166 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Highly Sensitive Colorimetric Assay for Determining Fe3+ Based on Gold Nanoparticles Conjugated with Glycol Chitosan.

Kim Kyungmin K Nam Yun-Sik YS Lee Yeonhee Y Lee Kang-Bong KB

Journal of analytical methods in chemistry 20170523

A highly sensitive and simple colorimetric assay for the detection of Fe3+ ions was developed using gold nanoparticles (AuNPs) conjugated with glycol chitosan (GC). The Fe3+ ion coordinates with the oxygen atoms of GC in a hexadentate manner (O-Fe3+-O), decreasing the interparticle distance and inducing aggregation. Time-of-flight secondary ion mass spectrometry showed that the bound Fe3+ was coordinated to the oxygen atoms of the ethylene glycol in GC ...[more]

PMID: 28630783

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Project description:Glycol chitosan (GC) is a hydrophilic chitosan derivative, known for its aqueous solubility. Previously, we have demonstrated the feasibility of preparing injectable, enzymatically crosslinked hydrogels from HPP [3-(4-Hydroxyphenyl)-propionic acid (98%)]-modified GC. However, HPP-GC gels showed very slow degradation, which presents challenges as an in vivo protein delivery vehicle. This study reports the potential of acetylated HPP-GC hydrogels as a biodegradable hydrogel platform for sustained protein delivery. Enzymatic crosslinking was used to prepare injectable, biodegradable hydrogels from HPP-GC with various degrees of acetylation (DA). The acetylated polymers were characterized using Fourier transform infrared and nuclear magnetic resonance spectroscopy. Rheological methods were used to characterize the mechanical behavior of the hydrogels. In vitro degradation and protein release were performed in the presence and absence of lysozyme. In vivo degradation was studied using a mouse subcutaneous implantation model. Finally, two hydrogel formulations with distinct in vitro/in vivo degradation and in vitro protein release were evaluated in 477-SKH1-Elite mice using live animal imaging to understand in vivo protein release profiles. The lysozyme-mediated degradation of the gels was demonstrated in vitro and the degradation rate was found to be dependent on the DA of the polymers. In vivo degradation study further confirmed that gels formed from polymers with higher DA degraded faster. In vitro protein release demonstrated the feasibility to achieve lysozyme-mediated protein release from the gels and that the rate of protein release can be modulated by varying the DA. In vivo protein release study further confirmed the feasibility to achieve differential protein release by varying the DA. The feasibility to develop degradable enzymatically crosslinked GC hydrogels is demonstrated. Gels with a wide spectrum of degradation time ranging from less than a week and more than 6 weeks can be developed using this approach. The study also showed the feasibility to fine tune in vivo protein release by modulating HPP-GC acetylation. The hydrogel platform therefore holds significant promise as a protein delivery vehicle for various biomedical and regenerative engineering applications. Impact statement The study describes the feasibility to develop a novel enzyme sensitive biodegradable and injectable hydrogel, where in the in vivo degradation rate and protein release profile can be modulated over a wide range. The described hydrogel platform has the potential to develop into a clinically relevant injectable and tunable protein delivery vehicle for a wide range of biomedical applications.

Dataset Information

Highly Sensitive Colorimetric Assay for Determining Fe³⁺ Based on Gold Nanoparticles Conjugated with Glycol Chitosan.

Publications

Highly Sensitive Colorimetric Assay for Determining Fe<sup>3+</sup> Based on Gold Nanoparticles Conjugated with Glycol Chitosan.

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