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High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats.

ABSTRACT: Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.

SUBMITTER: Regina A

PROVIDER: S-EPMC1450120 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature

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High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats.

Regina Ahmed A Bird Anthony A Topping David D Bowden Sarah S Freeman Judy J Barsby Tina T Kosar-Hashemi Behjat B Li Zhongyi Z Rahman Sadequr S Morell Matthew M

Proceedings of the National Academy of Sciences of the United States of America 20060228 10

Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose ...[more]

PMID: 16537443

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