Ontology highlight
ABSTRACT:
SUBMITTER: Sarafianos SG
PROVIDER: S-EPMC145536 | biostudies-literature | 2001 Mar
REPOSITORIES: biostudies-literature
Sarafianos S G SG Das K K Tantillo C C Clark A D AD Ding J J Whitcomb J M JM Boyer P L PL Hughes S H SH Arnold E E
The EMBO journal 20010301 6
We have determined the 3.0 A resolution structure of wild-type HIV-1 reverse transcriptase in complex with an RNA:DNA oligonucleotide whose sequence includes a purine-rich segment from the HIV-1 genome called the polypurine tract (PPT). The PPT is resistant to ribonuclease H (RNase H) cleavage and is used as a primer for second DNA strand synthesis. The 'RNase H primer grip', consisting of amino acids that interact with the DNA primer strand, may contribute to RNase H catalysis and cleavage spec ...[more]