Ontology highlight
ABSTRACT:
SUBMITTER: Hayes MJ
PROVIDER: S-EPMC1456940 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Hayes Matthew J MJ Shao Dongmin D Bailly Maryse M Moss Stephen E SE
The EMBO journal 20060406 9
Annexin 2 is a ubiquitous Ca(2+)-binding protein that is essential for actin-dependent vesicle transport. Here, we show that in spontaneously motile cells annexin 2 is concentrated in dynamic actin-rich protrusions, and that depletion of annexin 2 using siRNA leads to the accumulation of stress fibres and loss of protrusive and retractile activity. Cells co-expressing annexin 2-CFP and actin-YFP exhibit Ca(2+)-dependent fluorescense resonance energy transfer throughout the cytoplasm and in membr ...[more]