Ontology highlight
ABSTRACT:
SUBMITTER: Kubo S
PROVIDER: S-EPMC1472425 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Kubo Shuji S Seleme Maria Del Carmen MC Soifer Harris S HS Perez José Luis Garcia JL Moran John V JV Kazazian Haig H HH Kasahara Noriyuki N
Proceedings of the National Academy of Sciences of the United States of America 20060512 21
Whether long interspersed element-1 (L1 or LINE-1) retrotransposition can occur in quiescent, nondividing, and/or terminally differentiated somatic cells has remained an unanswered fundamental question in human genetics. Here, we used a ubiquitously active phosphoglycerate kinase-1 promoter to drive the expression of a highly active human L1 element from an adenovirus-L1 hybrid vector. This vector system achieved retrotransposition in up to 91% of actively growing immortalized cells, and we demo ...[more]