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Ordered assembly of the duplicating Golgi in Trypanosoma brucei.

ABSTRACT: The new Golgi in the protozoan parasite Trypanosoma brucei grows near to the old and adjacent to the growing new endoplasmic reticulum exit site. Growth is now shown to be at least a two-stage process, in which a representative matrix marker (GRASP) and enzyme (GntB) are delivered to the site of assembly, followed approximately 10 min later by a COPI component (epsilon-COP) and a trans-Golgi network (TGN) marker (GRIP70). A secretory cargo marker (signal sequence-YFP) appeared early near the new endoplasmic reticulum exit site but did not enter the Golgi until the second stage. Together these data suggest that structural and enzymatic components of the new Golgi stack are laid down first, followed by those needed to move and sort the cargo passing through it.

SUBMITTER: Ho HH

PROVIDER: S-EPMC1472504 | biostudies-literature | 2006 May

REPOSITORIES: biostudies-literature

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Ordered assembly of the duplicating Golgi in Trypanosoma brucei.

Ho Helen H HH He Cynthia Y CY de Graffenried Christopher L CL Murrells Lindsay J LJ Warren Graham G

Proceedings of the National Academy of Sciences of the United States of America 20060503 20

The new Golgi in the protozoan parasite Trypanosoma brucei grows near to the old and adjacent to the growing new endoplasmic reticulum exit site. Growth is now shown to be at least a two-stage process, in which a representative matrix marker (GRASP) and enzyme (GntB) are delivered to the site of assembly, followed approximately 10 min later by a COPI component (epsilon-COP) and a trans-Golgi network (TGN) marker (GRIP70). A secretory cargo marker (signal sequence-YFP) appeared early near the new ...[more]

PMID: 16672362

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