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Polo-like kinase is required for Golgi and bilobe biogenesis in Trypanosoma brucei.

ABSTRACT: A bilobed structure marked by TbCentrin2 regulates Golgi duplication in the protozoan parasite Trypanosoma brucei. This structure must itself duplicate during the cell cycle for Golgi inheritance to proceed normally. We show here that duplication of the bilobed structure is dependent on the single polo-like kinase (PLK) homologue in T. brucei (TbPLK). Depletion of TbPLK leads to malformed bilobed structures, which is consistent with an inhibition of duplication and an increase in the number of dispersed Golgi structures with associated endoplasmic reticulum exit sites. These data suggest that the bilobe may act as a scaffold for the controlled assembly of the duplicating Golgi.

SUBMITTER: de Graffenried CL

PROVIDER: S-EPMC2364693 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature

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Polo-like kinase is required for Golgi and bilobe biogenesis in Trypanosoma brucei.

de Graffenried Christopher L CL Ho Helen H HH Warren Graham G

The Journal of cell biology 20080428 3

A bilobed structure marked by TbCentrin2 regulates Golgi duplication in the protozoan parasite Trypanosoma brucei. This structure must itself duplicate during the cell cycle for Golgi inheritance to proceed normally. We show here that duplication of the bilobed structure is dependent on the single polo-like kinase (PLK) homologue in T. brucei (TbPLK). Depletion of TbPLK leads to malformed bilobed structures, which is consistent with an inhibition of duplication and an increase in the number of d ...[more]

PMID: 18443217

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