Ontology highlight
ABSTRACT:
SUBMITTER: Moissoglu K
PROVIDER: S-EPMC1474787 | biostudies-literature | 2006 Jun
REPOSITORIES: biostudies-literature
Moissoglu Konstadinos K Slepchenko Boris M BM Meller Nahum N Horwitz Alan F AF Schwartz Martin A MA
Molecular biology of the cell 20060405 6
The small GTPase Rac cycles between the membrane and the cytosol as it is activated by nucleotide exchange factors (GEFs) and inactivated by GTPase-activating proteins (GAPs). Solubility in the cytosol is conferred by binding of Rac to guanine-nucleotide dissociation inhibitors (GDIs). To analyze the in vivo dynamics of Rac, we developed a photobleaching method to measure the dissociation rate constant (k(off)) of membrane-bound GFP-Rac. We find that k(off) is 0.048 s(-1) for wtRac and approxima ...[more]