Ontology highlight
ABSTRACT:
SUBMITTER: Wada Y
PROVIDER: S-EPMC14765 | biostudies-literature | 2000 Jan
REPOSITORIES: biostudies-literature
Wada Y Y Hamasaki T T Satir P P
Molecular biology of the cell 20000101 1
In microtubule (MT) translocation assays, using colloidal gold particles coupled to monoclonal tubulin antibodies to mark positions along MTs, we found that relative motion is possible between the gold particle and an MT, gliding on dynein or kinesin. Such motion evidently occurred by an affinity release and rebinding mechanism that did not require motor activity on the particle. As the MTs moved, particles drifted to the trailing edge of the MT and then were released. Sometimes the particles tr ...[more]