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Protein kinase Darkener of apricot and its substrate EF1? regulate organelle transport along microtubules.

ABSTRACT: Regulation of organelle transport along microtubules is important for proper distribution of membrane organelles and protein complexes in the cytoplasm. RNAi-mediated knockdown in cultured Drosophila S2 cells demonstrates that two microtubule-binding proteins, a unique isoform of Darkener of apricot (DOA) protein kinase, and its substrate, translational elongation factor EF1?, negatively regulate transport of several classes of membrane organelles along microtubules. Inhibition of transport by EF1? requires its phosphorylation by DOA on serine 294. Together, our results indicate a new role for two proteins that have not previously been implicated in regulation of the cytoskeleton. These results further suggest that the biological role of some of the proteins binding to the microtubule track is to regulate cargo transport along these tracks.

SUBMITTER: Serpinskaya AS

PROVIDER: S-EPMC3874782 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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Protein kinase Darkener of apricot and its substrate EF1γ regulate organelle transport along microtubules.

Serpinskaya Anna S AS Tuphile Karine K Rabinow Leonard L Gelfand Vladimir I VI

Journal of cell science 20131025 Pt 1

Regulation of organelle transport along microtubules is important for proper distribution of membrane organelles and protein complexes in the cytoplasm. RNAi-mediated knockdown in cultured Drosophila S2 cells demonstrates that two microtubule-binding proteins, a unique isoform of Darkener of apricot (DOA) protein kinase, and its substrate, translational elongation factor EF1γ, negatively regulate transport of several classes of membrane organelles along microtubules. Inhibition of transport by E ...[more]

PMID: 24163433

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