Ontology highlight
ABSTRACT:
SUBMITTER: Buchwald G
PROVIDER: S-EPMC1478191 | biostudies-literature | 2006 Jun
REPOSITORIES: biostudies-literature
Buchwald Gretel G van der Stoop Petra P Weichenrieder Oliver O Perrakis Anastassis A van Lohuizen Maarten M Sixma Titia K TK
The EMBO journal 20060518 11
Polycomb group proteins Ring1b and Bmi1 (B-cell-specific Moloney murine leukaemia virus integration site 1) are critical components of the chromatin modulating PRC1 complex. Histone H2A ubiquitination by the PRC1 complex strongly depends on the Ring1b protein. Here we show that the E3-ligase activity of Ring1b on histone H2A is enhanced by Bmi1 in vitro. The N-terminal Ring-domains are sufficient for this activity and Ring1a can replace Ring1b. E2 enzymes UbcH5a, b, c or UbcH6 support this activ ...[more]