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The E3 ubiquitin ligase activity of RING1B is not essential for early mouse development.

ABSTRACT: Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other's binding but suggest that the key functions of PRC1 lie beyond the enzymatic capabilities of RING1B.

SUBMITTER: Illingworth RS

PROVIDER: S-EPMC4579347 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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The E3 ubiquitin ligase activity of RING1B is not essential for early mouse development.

Illingworth Robert S RS Moffat Michael M Mann Abigail R AR Read David D Hunter Chris J CJ Pradeepa Madapura M MM Adams Ian R IR Bickmore Wendy A WA

Genes & development 20150901 18

Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other' ...[more]

PMID: 26385961

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