Ontology highlight
ABSTRACT:
SUBMITTER: de Koning M
PROVIDER: S-EPMC1479195 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
de Koning Maurits M Quint Wim W Struijk Linda L Kleter Bernhard B Wanningen Patrick P van Doorn Leen-Jan LJ Weissenborn Sönke Jan SJ Feltkamp Mariet M ter Schegget Jan J
Journal of clinical microbiology 20060501 5
Human papillomavirus can be detected by amplification of viral DNA. A novel one-step PCR (PM-PCR) was evaluated for amplification of a 117-bp fragment from the E1 region. It permitted ultrasensitive detection of all 25 known human papillomavirus genotypes from the beta-papillomavirus genus. The intra- and intertypic sequence variations of the 77-bp interprimer region were studied. Genotype-specific probes as well as general probes were selected for the 25 established beta-papillomavirus types, a ...[more]