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Use of real-time PCR to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" DNA.

ABSTRACT: A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.

SUBMITTER: Tasker S 

PROVIDER: S-EPMC149582 | biostudies-literature | 2003 Jan

REPOSITORIES: biostudies-literature

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Use of real-time PCR to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" DNA.

Tasker Séverine S   Helps Chris R CR   Day Michael J MJ   Gruffydd-Jones Tim J TJ   Harbour Dave A DA  

Journal of clinical microbiology 20030101 1

A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and "Candidatus Mycoplasma haemominutum" in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species. ...[more]

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