Ontology highlight
ABSTRACT:
SUBMITTER: Neu T
PROVIDER: S-EPMC150095 | biostudies-literature | 2003 Mar
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20030301 3
Derivatives of a cryptic plasmid from Lactobacillus curvatus showed temperature-sensitive replication in thermophilic lactobacilli. The thermosensitive replicon was used to construct the new delivery vector pTN1, which allows site-specific replacement of chromosomal DNA sequences. pTN1 carries an erythromycin resistance marker suitable for selection of single-copy integrants and replicates readily at 35 degrees C, whereas replication is efficiently shut down at 42 degrees C. To demonstrate the f ...[more]