Ontology highlight
ABSTRACT:
SUBMITTER: Bui MH
PROVIDER: S-EPMC152482 | biostudies-literature | 2003 Apr
REPOSITORIES: biostudies-literature
Bui Mai-Ha MH Stone Gregory G GG Nilius Angela M AM Almer Laurel L Flamm Robert K RK
Antimicrobial agents and chemotherapy 20030401 4
We have developed a PCR-oligonucleotide ligation assay to rapidly identify base substitutions in topoisomerase genes that are associated with quinolone resistance in clinical isolates of Streptococcus pneumoniae. Thirty-seven strains for which the ciprofloxacin MICs were >/=4 micro g/ml and 16 strains for which the MICs were </=2 micro g/ml were assayed. Compared with sequence data, the assay correctly identified the DNA bases that encoded amino acids at the four positions most commonly associat ...[more]