Ontology highlight
ABSTRACT:
SUBMITTER: Bunting KA
PROVIDER: S-EPMC152875 | biostudies-literature | 2003 Mar
REPOSITORIES: biostudies-literature
Bunting Karen A KA Roe S Mark SM Headley Anthony A Brown Tom T Savva Renos R Pearl Laurence H LH
Nucleic acids research 20030301 6
Very-short-patch repair (Vsr) enzymes occur in a variety of bacteria, where they initiate nucleotide excision repair of G:T mismatches arising by deamination of 5-methyl-cytosines in specific regulatory sequences. We have now determined the structure of the archetypal dcm-Vsr endonuclease from Escherichia coli bound to the cleaved authentic hemi-deaminated/hemi-methylated dcm sequence 5'-C-OH-3' 5'-p-T-p-A-p-G-p-G-3'/3'-G-p-G-p-T-p(Me5)C-p-C formed by self-assembly of a 12mer oligonucleotide int ...[more]