Unknown

Dataset Information

0

The structural basis of damaged DNA recognition and endonucleolytic cleavage for very short patch repair endonuclease.


ABSTRACT: Endonucleases in DNA repair must be able to recognize damaged DNA as well as cleave the phosphodiester backbone. These functional prerequisites are manifested in very short patch repair (Vsr) endonuclease through a common endonuclease topology that has been tailored for recognition of TG mismatches. Structural and biochemical comparison with type II restriction enzymes illustrates how Vsr resembles these endonucleases in overall topology but also how Vsr diverges in terms of the detailed catalytic mechanism. A histidine and two metal-water clusters catalyze the phosphodiester cleavage. The mode of DNA damage recognition is also unique to Vsr. All other structurally characterized DNA damage-binding enzymes employ a nucleotide flipping mechanism for substrate recognition and for catalysis. Vsr, on the other hand, recognizes the TG mismatch as a wobble base pair and penetrates the DNA with three aromatic residues on one side of the mismatch. Thus, Vsr endonuclease provides important counterpoints in our understanding of endonucleolytic mechanisms and of damaged DNA recognition.

SUBMITTER: Tsutakawa SE 

PROVIDER: S-EPMC55919 | biostudies-literature | 2001 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

The structural basis of damaged DNA recognition and endonucleolytic cleavage for very short patch repair endonuclease.

Tsutakawa S E SE   Morikawa K K  

Nucleic acids research 20010901 18


Endonucleases in DNA repair must be able to recognize damaged DNA as well as cleave the phosphodiester backbone. These functional prerequisites are manifested in very short patch repair (Vsr) endonuclease through a common endonuclease topology that has been tailored for recognition of TG mismatches. Structural and biochemical comparison with type II restriction enzymes illustrates how Vsr resembles these endonucleases in overall topology but also how Vsr diverges in terms of the detailed catalyt  ...[more]

Similar Datasets

| S-EPMC152875 | biostudies-literature
| S-EPMC2807027 | biostudies-literature
| S-EPMC213244 | biostudies-other
| S-EPMC4188408 | biostudies-literature
| S-EPMC2976803 | biostudies-literature
| S-EPMC5278635 | biostudies-literature
| S-EPMC8256696 | biostudies-literature
| S-EPMC4357722 | biostudies-literature
| S-EPMC7504742 | biostudies-literature
| S-EPMC2761273 | biostudies-literature