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Specific identification of Listeria welshimeri and Listeria monocytogenes by PCR assays targeting a gene encoding a fibronectin-binding protein.

ABSTRACT: We have cloned and sequenced a Listeria welshimeri DNA fragment homologous to the previously described fibronectin-binding protein-encoding gene (fbp) of Listeria monocytogenes (P. Gilot, Y. Jossin, and J. Content, J. Med. Microbiol., 49:887-896, 2000). This L. welshimeri DNA fragment expresses a 24.8-kDa protein that binds to human fibronectin. Based on the fbp sequences, we developed novel PCR assays for the identification of L. welshimeri and L. monocytogenes.

SUBMITTER: Gilot P

PROVIDER: S-EPMC153408 | biostudies-literature | 2002 Feb

REPOSITORIES: biostudies-literature

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Specific identification of Listeria welshimeri and Listeria monocytogenes by PCR assays targeting a gene encoding a fibronectin-binding protein.

Gilot Philippe P Content Jean J

Journal of clinical microbiology 20020201 2

We have cloned and sequenced a Listeria welshimeri DNA fragment homologous to the previously described fibronectin-binding protein-encoding gene (fbp) of Listeria monocytogenes (P. Gilot, Y. Jossin, and J. Content, J. Med. Microbiol., 49:887-896, 2000). This L. welshimeri DNA fragment expresses a 24.8-kDa protein that binds to human fibronectin. Based on the fbp sequences, we developed novel PCR assays for the identification of L. welshimeri and L. monocytogenes. ...[more]

PMID: 11826001

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